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The Shareable Instrumentation Facility

 

Core I provides TU researchers with the necessary equipment to conduct flexible and thorough studies. This facility allows on-site use of an array of equipment that would otherwise be inaccessible to individual researchers, due to budgetary constraints. The instrumentation is divided between two locations: Williams-Bowie Research Building and Carver Research Building. Additionally, two full-time employees ensure that all users are properly trained and that equipment stays in a ready-to-use state.  


Resources within this core offer the following capabilities: Digital Wide-field and Confocal Microscopy, Gene Expression Analysis, Flow Cytometry, Microplate Reading (Absorbance, Fluorescence and Luminescence), Gel Imaging, Tissue Culture, HPLC, GC/MS, Pulsed Field Gel Electrophoresis, Electroporation, Ultra High-Speed Centrifugation, Film Processing and InVivo Imaging. 

 


Important Announcements:

  • Facility access is controlled and monitored using electronic keypad locks.  Users can request a unique 4-digit access code after being trained on equipment of interest.  Requests are made by completing and submitting the Access Acknowledgment-fillable.pdf to Jason White
  • Equipment use is scheduled via the Core's Google Calendar.  Login details are provided during instrument training.   
  • A complete listing of Core I facilities and resources has been compiled. TU RCMI Facilities and Resources 2014.docx 
  • All presentations, posters and publications containing data generated using this facility should cite the RCMI Core grant number (G12MD007585-23); moreover, publications must be placed into PubMed Central (PMC).  Once entered into PMC, the PMID and PMCID numbers are evidence of core productivity.  Yearly, NIH uses these numbers to determine the impact of CBR/RCMI Core facilities, for the purpose of assessing the level of future funding.  More information can be found at the following sites: PubMed CentralNIH Manuscript Submission System, and NIH Public Access Policy.

Available facilities include:



Digital Imaging   

Confocal and widefield microscopes are located in the Carver Research Building (CRB) in room 33.  
  • Two confocal fluorescent microscopes 
    • Olympus Laser Scanning Confocal 
      • Operated using Fluoview software
    • Olympus Spinning Disk Confocal
      • Operated using MetaMorph software
      • Capable of imaging live cell experiments
  • Two widefield fluorescent microscopes
    • Leica Upright
    • Leica Inverted
    • Both scopes use CellSens software



              
Widefield microscopes and a immunohistochemistry (IHC) microscope are located in Williams-Bowie Hall in room 2055.  
  • Two Olympus widefield fluorescent microscopes 
    • One inverted and one upright 
    • Both scopes use CellSens software 
  • One IHC microscope
    • Upright
    • Uses CellSens Dimension software

  


An IVIS Lumina XR is housed in the Animal Facility (1st floor of WB Hall). 
  • Multimodal imaging
    • Fluorescence, bioluminescence, x-ray and photography
  • Compatible with rats, mice, cell cultures, bacterial cultures, and microplates
  • Operated using Living Image software
 
                                    


Three flow cytometers are available. 
  • A Sony iCyt EC800 system is located in WB Hall room 2055
    • Operated using EC800 Software
  • A FACSCalibur system is located in WB Hall room 2055.
    • Operated using FlowJo software
  • An Accuri C6 is located in Carver Research Building room 31. 
    • Operated using CFlow software
                         

Gene Expression 

 
  Two Real-Time PCR systems are located in WB Hall room 2017. 
  • One Stratagene Mx3000P
  • One Stratagene Mx3005P
  • Both systems are operated using MXPro software
  • Two Eppendorf thermocyclers are also available within the same space
                                
 
Five plate readers are distributed between WB Hall and CRB.
  • WB Hall room 2017
    • One BioWave XS (absorbance)
    • One Synergy 2 (luminescence)
    • Both operated using Gen5 2.0 software
  • CRB room 19
    • One Synergy HT (fluorescence and absorbance)
      • Operated using Gen5 2.0 software
  • CRB room 31
    • One ThermoMax (absorbance)
    • One SpectroMax Gemini EM (luminescence)
    • Both are operated using SoftMax Pro software
 
                                                          
 Chromatography  
One HPLC is in WB Hall room 2025.
  • Operated using Karat 32 software
                                                                                            


Cell Culture

Two cell culture facilities are also available to users.
  • One is located in WB Hall room 2023
  • One is located in CRB room 27

Animal Facility and Pathology Services

The College has a modern animal facility capable of accommodating from rodents to large animals and well-organized Comparative Medicine Resource Center (CMRC) for research involving laboratory animals and small ruminants. The facility provides all services to researchers who have projects approved for animal use. The animal facility serves the biomedical research community in the university and is fully equipped with, among others, offices, garment changing areas, animal isolation/quarantine, housing rooms (for both conventional and special accommodations), and rooms for feed storage, cold holding and necropsy. A designated attending veterinarian, a facility manager, a center director and adequate non-technical support personnel are available.

 Pathology services are provided to the clinical and research community on a fee-for-service basis. Fully equipped and man-powered tissue processing service facility is located in Williams-Bowie building. One board-certified veterinary pathologist and three faculty (with a Doctorate degree in Anatomical Pathology) are available for consultation.