John P. Davidson, Department Head and Associate Professor
Location: Armstrong Hall 201A
Office Phone: 334-727-8822
A comprehensive bioinformatics approach to examine how global gene expression is related to chromosome structure in Bacteria. We have obtained a temperature-sensitive gyrase mutant (gyrBts) which shows a dramatic decrease in the level of supercoiling in a reporter plasmid at the near-restrictive temperature. Alteration of expression of individual genes in the mutant vs normal strain is also observed at the near-restrictive temperature. We seek to compare global expression of genes of a mutant and normal strain by microarray analysis of the transcriptome. We further propose to compare transcriptomes of normal and mutant strains by deep sequencing analysis.
1. John P. Davidson and L. S. Williams. 1979. Regulation of Isoleucine-Valine Biosynthesis in Salmonella typhimurium: The Effect of hisU on Repression Control. J. Mol.Biol.27:229-235.
2. John P. Davidson and L. S. Williams. 1979. Relaxed Control of RNA Synthesis during Nutritional Shiftdowns of a hisU Mutant of Salmonella typhimurium. Biochem. Biophys. Res. Commun. 88:682-687.
3. Wilson, Donella J., J. P. Davidson and L. S. Williams. 1981. Anomalies in The Guanosine Polyphosphate Metabolism: Effect of the hisU Mutation. Biochem. Biophys. Res. Commun. 103:319-326
4. John P. Davidson, Donella J. Wilson and L. S. Williams. 1982. The Role of A hisU Gene in The Control of Stable RNA Synthesis in Salmonella typhimurium. J. Mol. Biol. 157:237-264.
5. John P. Davidson and Donella J. Wilson. 1991. Evidence for Isoleucine as A Positive Effector of The ilvBN Operon of in Salmonella typhimurium. Biochem. Biophys. Res. Commun. 178:934-939
6. Davidson, John P.,J. Chiu, W. Session, V. Suppiramaniam E. Munir. 1995. The Effect of Temperature on DNA Supercoiling in A gyrB Mutant of Salmonellla typhimurium. Journal of Cellular Biochemistry, Supplement 19A.