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Tuskegee University Veterinary Medical Teaching Hospital

HISTOPATHOLOGY LABORATORY

The histopathology laboratory is capable of performing routine as well as a variety of special stains on fixed tissues. The laboratory is fully automated to achieve rapid processing and staining of microslides. Special staining procedures utilize chemical compounds on tissue sections to characterize cell types and identify tissue components. The following are some of the special stains and histochemical methods available:

* PAS (periodic acid-Shiff reagent) - Stains glycogen and mucins, differentiated by treatment with and without diastase.

* Mucicarmine - For the demostration of epithelial mucins

* Trichrome stain (Masson’s method)- For connective tissue

* Tissue Gram’s Stain (Modified Brown and Brenn)

* Acid-Fast Stain (Ziehl-Neelsen) -For demonstration of Mycobacterium

* Warthin-Starry - For demonstration of spirochetes

* Giemsa Stain - For demostration of mast cells

* Gomori’s Methanamine Silver Stain (GMS) - for demonstration of fungi

* Microincineration for the demonstration of calcium oxalate in tissue sections

Specimen Submission (necropsy and biopsy specimens)

Specimens submitted for histopathological examination should be fixed in 10% neutral buffered formalin. Tissues should be sectioned not more than ¼ inches in thickness and placed in fixative 10x the volume of tissue samples. Do not freeze tissues. Specimen jars should be clearly labeled, sealed tightly, and boxed with shock absorbent material for shipment. Clients in areas where freezing is possible should add 10% ethyl alcohol to formalin to prevent freezing artifacts in winter.

Light Microscopy

With the aid of the light microscope, histopathological evaluation of tissue sections are performed by experienced veterinary pathologists with many years of experience in diagnostic and toxicological pathology. Pathologists are available to help clients in developing necropsy and histopathological protocols for experimental studies. In addition, our pathologists welcome collaboration as co-investigators in interdisciplinary research.